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Listeria sp.
Králíková, Lenka ; Vejsová, Marcela (advisor) ; Janďourek, Ondřej (referee)
Author: Lenka Králíková Title: Listeria spp. Bachelor thesis Charles University in Prague, Faculty of Pharmacy, Hradec Králové Field of study: Medical Labolatory Background: The bachelor thesis aims to describe Listeria spp. Describe the division of genus, morphology and laboratory diagnostics. More attention is paid to Listeria monocytogenes as it is a major source of infection in humans. Another aim is to describe the occurrence of L. monocytogenes in food, its antigenic structure, pathogenesis and the disease it causes. Main findings: Listeria monocytogenes is a bacterium present everywhere. It was found in fresh water, clay, plant material and waste water. The main source of infection for humans is contaminated food. They are most often found in unpasteurized milk, matured cheeses or poorly cooked meat or vegetables. Bacteria causes a disease called listeriosis, which is especially dangerous for newborns, elderly people, pregnant women and immunocompromised individuals. The disease occurs rather sporadically. There was confirmed from 30 to 37 cases per year in the Czech Republic between years 2008-2017. There was also an epidemic in Africa in 2017-2018, it was the largest epidemic in the world. In the event of infection, both the laboratory and the physician are obliged to report the positivity...
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Extending of shelf life of chilled fish products
PFLUG, Róbert
This diploma thesis was focused on the possibilities of extending the shelf-life of fish products by dipping containing seven commercial additives. ANTIBAK, MIC STAB, Bakont, SEA-F75, Misocarine LR, SAFE A Plus and AMX liquid. The effectiveness of these substances on the extending of shelf-life was evaluated on the basis of tests of TVC (total viable count), level of fat and protein oxidation, determination of nutritional parameters of muscle, and finaly sensory analysis. Experimental species were 2 important commodities for the Czech aquaculture rainbow trout (Oncorhynchus mykiss) and common carp (Cyprinus carpio). The project was divided into 6 sub-stages. During testing was the initial number of aditives (7) limited by stepwise selection for 2 preparations. Namely Bakont and AMX liquid, which were tested further on trout (filet with skin and scales) and carp (filets with skin without scales) chilled bulk and chilled packaged under vakuum. For trout was studied antimicrobial effect of dipping on eviscerated fish with the head with- or without gills. Treated trout fillets in bulk and vakuum-packed showed significantly less abundance of muscle mikroflora. In the case of carp fillets chilled bulk we can not say that the bath had influence on the CMP in meat. However, the combination of dipping and vakuum packaging was singnificantly different between the control and product Bakont. AMX liquid was not applied in this case in sufficient dose or in sufficient time to carp muscle. A positive finding is that the application of the aditives to the product "eviscerated trout with head" it does not matter, whether the gills are left in fish or not. However, in all cases the analysis of the presence of pathogens Escherichia coli, Salmonella spp. or Listeria monocytogenes were negative. From the results of sensory analysis can be concluded, that the substances contained in aditives are not reflected in the sensory properties of tested fish.
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